sox9 primers ppr53329a Search Results


afp ppr44288a  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation afp ppr44288a
Genes involved with HBT in organoid culture over a time course of 21 days. mRNA levels of (A) HNF-1β, (B) <t>Sox9,</t> (C) Afp, and (D) Fxr as assessed by qRT-PCR and expressed as fold change relative to GAPDH. Significantly different from D3 time point: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Afp Ppr44288a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ck19 primers ppr44322a  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation ck19 primers ppr44322a
Genes involved with HBT in organoid culture over a time course of 21 days. mRNA levels of (A) HNF-1β, (B) <t>Sox9,</t> (C) Afp, and (D) Fxr as assessed by qRT-PCR and expressed as fold change relative to GAPDH. Significantly different from D3 time point: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
Ck19 Primers Ppr44322a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ck19 primers ppr44322a - by Bioz Stars, 2026-07
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hes1 ppr46895c  (SuperArray Bioscience Corporation)
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SuperArray Bioscience Corporation hes1 ppr46895c
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) <t>Notch2,</t> (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Hes1 Ppr46895c, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hes1 ppr46895c - by Bioz Stars, 2026-07
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oct4 ppr59727a  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation oct4 ppr59727a
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) <t>Notch2,</t> (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Oct4 Ppr59727a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oct4 ppr59727a - by Bioz Stars, 2026-07
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notch1 primers ppr47971a  (SuperArray Bioscience Corporation)
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SuperArray Bioscience Corporation notch1 primers ppr47971a
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) <t>Notch2,</t> (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Notch1 Primers Ppr47971a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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notch1 primers ppr47971a - by Bioz Stars, 2026-07
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notch2 ppr51608a  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation notch2 ppr51608a
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) <t>Notch1,</t> (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Notch2 Ppr51608a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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notch2 ppr51608a - by Bioz Stars, 2026-07
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sox9 primers ppr53329a  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation sox9 primers ppr53329a
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) <t>Notch1,</t> (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Sox9 Primers Ppr53329a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sox9 primers ppr53329a - by Bioz Stars, 2026-07
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yap1 (ppr54845a)  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation yap1 (ppr54845a)
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) <t>Notch1,</t> (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Yap1 (Ppr54845a), supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gapdh primers ppr06557b  (SuperArray Bioscience Corporation)
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SuperArray Bioscience Corporation gapdh primers ppr06557b
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) <t>Notch1,</t> (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Gapdh Primers Ppr06557b, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hepatocyte nuclear factor-1b primers ppr45380a  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation hepatocyte nuclear factor-1b primers ppr45380a
Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) <t>Notch1,</t> (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.
Hepatocyte Nuclear Factor 1b Primers Ppr45380a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hepatocyte nuclear factor-1b primers ppr45380a - by Bioz Stars, 2026-07
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hnf-4α ppr49773a  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation hnf-4α ppr49773a
<t>Oct4</t> expression during HBT over a time course of 21 days in organoid culture. (A) Representative photomicrographs of Oct4 immunohistochemistry in organoid culture tissue samples. –ve represents “no primary antibody” control. Thin arrows indicate Oct4+ nuclei undergoing HBT. Thick arrows indicate Oct4− nuclei that retain hepatocytic phenotype. Original magnification: 200×. (B) Representative blots of polymerase chain reaction (PCR) product and (C) mRNA levels assessed by quantitative reverse transcription (qRT)PCR) and expressed as fold change relative to GAPDH. Significantly different from day 3 (D3) time point: **p ≤ 0.01, ***p ≤ 0.001.
Hnf 4α Ppr49773a, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Genes involved with HBT in organoid culture over a time course of 21 days. mRNA levels of (A) HNF-1β, (B) Sox9, (C) Afp, and (D) Fxr as assessed by qRT-PCR and expressed as fold change relative to GAPDH. Significantly different from D3 time point: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Genes involved with HBT in organoid culture over a time course of 21 days. mRNA levels of (A) HNF-1β, (B) Sox9, (C) Afp, and (D) Fxr as assessed by qRT-PCR and expressed as fold change relative to GAPDH. Significantly different from D3 time point: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Quantitative RT-PCR

Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Expressing, Inhibition, Quantitative RT-PCR, Control

Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Expressing, Inhibition, Quantitative RT-PCR, Control

Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Expressing, Inhibition, Quantitative RT-PCR, Control

Oct4 expression during HBT over a time course of 21 days in organoid culture. (A) Representative photomicrographs of Oct4 immunohistochemistry in organoid culture tissue samples. –ve represents “no primary antibody” control. Thin arrows indicate Oct4+ nuclei undergoing HBT. Thick arrows indicate Oct4− nuclei that retain hepatocytic phenotype. Original magnification: 200×. (B) Representative blots of polymerase chain reaction (PCR) product and (C) mRNA levels assessed by quantitative reverse transcription (qRT)PCR) and expressed as fold change relative to GAPDH. Significantly different from day 3 (D3) time point: **p ≤ 0.01, ***p ≤ 0.001.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Oct4 expression during HBT over a time course of 21 days in organoid culture. (A) Representative photomicrographs of Oct4 immunohistochemistry in organoid culture tissue samples. –ve represents “no primary antibody” control. Thin arrows indicate Oct4+ nuclei undergoing HBT. Thick arrows indicate Oct4− nuclei that retain hepatocytic phenotype. Original magnification: 200×. (B) Representative blots of polymerase chain reaction (PCR) product and (C) mRNA levels assessed by quantitative reverse transcription (qRT)PCR) and expressed as fold change relative to GAPDH. Significantly different from day 3 (D3) time point: **p ≤ 0.01, ***p ≤ 0.001.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Expressing, Immunohistochemistry, Control, Polymerase Chain Reaction, Reverse Transcription, Quantitative RT-PCR

Oct4 inhibition in organoid cultures. (A) Timeline for Oct4 inhibition using adenovirus in organoid culture. (B) Enhanced green fluorescent protein (eGFP) in hepatocytes infected with adenovirus containing short hairpin RNA (shRNA) for Oct4, multiplicity of infection (MOI) 5, >70% infection observed at day 14 in culture. Original magnification: 100×. (C–G) Analyzed from D21 samples treated with shRNA for Oct4 (AD-Oct4) or shRNA for scramble control (AD-Scr). (C) mRNA levels of Oct4 assessed by qRT-PCR and expressed as fold change relative to GAPDH. (D) Oct4 protein assessed by sELISA. mRNA levels as assessed by qRT-PCR and expressed as fold change relative to GAPDH of (E) biliary-specific marker HNF-1β, (F) hepatocyte-specific marker HNF-4α, and (G) biliary-specific marker CK19. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Oct4 inhibition in organoid cultures. (A) Timeline for Oct4 inhibition using adenovirus in organoid culture. (B) Enhanced green fluorescent protein (eGFP) in hepatocytes infected with adenovirus containing short hairpin RNA (shRNA) for Oct4, multiplicity of infection (MOI) 5, >70% infection observed at day 14 in culture. Original magnification: 100×. (C–G) Analyzed from D21 samples treated with shRNA for Oct4 (AD-Oct4) or shRNA for scramble control (AD-Scr). (C) mRNA levels of Oct4 assessed by qRT-PCR and expressed as fold change relative to GAPDH. (D) Oct4 protein assessed by sELISA. mRNA levels as assessed by qRT-PCR and expressed as fold change relative to GAPDH of (E) biliary-specific marker HNF-1β, (F) hepatocyte-specific marker HNF-4α, and (G) biliary-specific marker CK19. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Inhibition, Infection, shRNA, Control, Quantitative RT-PCR, Marker

Appearance of biliary cells from hepatocytes following Oct4 inhibition. Representative photomicrographs of biliary-specific marker HNF-1β following Oct4 inhibition in organoid culture tissue samples on day 21. (A–C) Treated with AD-Scr. (D–F) Treated with AD-Oct4. Thin arrows indicate HNF-1β+ biliary cells. Thick arrows indicate lack of HNF-1β+ biliary cells. Original magnification: 200× (A, B, D, and E) and 400× (C, F).

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Appearance of biliary cells from hepatocytes following Oct4 inhibition. Representative photomicrographs of biliary-specific marker HNF-1β following Oct4 inhibition in organoid culture tissue samples on day 21. (A–C) Treated with AD-Scr. (D–F) Treated with AD-Oct4. Thin arrows indicate HNF-1β+ biliary cells. Thick arrows indicate lack of HNF-1β+ biliary cells. Original magnification: 200× (A, B, D, and E) and 400× (C, F).

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Inhibition, Marker

Effect of Oct4 inhibition on hepatocytes. Representative photomicrographs of hepatocyte-specific HNF-4α following Oct4 inhibition in organoid culture tissue samples on day 21. (A, B) Treated with AD-Scr. (C, D) Treated with AD-Oct4. Thick arrows indicate HNF-4α− cells. Thin arrows indicate HNF-4α+ cells. Original magnification: 400×. Cell counts of hepatocytes and biliary epithelial cells following Oct4 inhibition. (E) Biliary-to-hepatocyte ratio and (F) number of hepatocytes. Significantly different from AD-Scr control: **p ≤ 0.01.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Effect of Oct4 inhibition on hepatocytes. Representative photomicrographs of hepatocyte-specific HNF-4α following Oct4 inhibition in organoid culture tissue samples on day 21. (A, B) Treated with AD-Scr. (C, D) Treated with AD-Oct4. Thick arrows indicate HNF-4α− cells. Thin arrows indicate HNF-4α+ cells. Original magnification: 400×. Cell counts of hepatocytes and biliary epithelial cells following Oct4 inhibition. (E) Biliary-to-hepatocyte ratio and (F) number of hepatocytes. Significantly different from AD-Scr control: **p ≤ 0.01.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Inhibition, Control

Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Journal: Gene Expression

Article Title: Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model

doi: 10.3727/105221617X15124876321401

Figure Lengend Snippet: Expression of genes associated with HBT following Oct4 inhibition. mRNA levels of (A) Notch1, (B) Notch2, (C) Sox9, (D) YAP1, and (E) Hes1 as assessed by qRT-PCR and expressed as fold change relative to GAPDH on day 21. (F) Representative blots of PCR product following Oct4 inhibition. Significantly different from AD-Scr control: *p ≤ 0.05, **p ≤ 0.01.

Article Snippet: Gene-specific primers used for rat were obtained from SuperArray Biosciences Corporation as follows: GAPDH (PPR06557B), Oct4 (PPR59727A), HNF-4α (PPR49773A), hepatocyte nuclear factor-1β (HNF-1β; PPR45380A), CK19 (PPR44322A), Sox9 (PPR53329A), Afp (PPR44288A), Notch1 (PPR47971A), Notch2 (PPR51608A), Hes1 (PPR46895C), and Yap1 (PPR54845A).

Techniques: Expressing, Inhibition, Quantitative RT-PCR, Control